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KMID : 0364820200560030297
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Korean Journal of Microbiology
2020 Volume.56 No. 3 p.297 ~ p.306
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Investigation on human milk oligosaccharide utilization and vitamin B biosynthesis by Bifidobacterium strains isolated from infant feces
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Jung Hye-Su
Kim Kyeong-Ryeol
Kim Kyung-Hyun
Kim Hong-Man
Lim Sang-Hyun
Chung Myung-Jun
Jeon Che-Ok
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Abstract
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Utilization of indigestible oligosaccharides and human milk oligosaccharide (HMO) and biosynthesis of vitamin B by four Bifidobacterium strains (B. longum subsp. infantis CBT BT1, B. bifidum CBT BF3, B. breve CBT BR3, and B. longum CBT BG7) harboring genes associated with fructooligosaccharide (FOS), galactooligosaccharide (GOS), and HMO, 2'-fucosyllactose and 3'-sialyllactose, metabolism and folate, nicotinate, and riboflavin synthesis, which were isolated from infant feces, were experimentally verified. Indigestible oligosaccharides, HMO, and vitamin B compounds were analyzed using LC-QTOF/ MS. All Bifidobacterium strains utilized GOS better than FOS and especially, B. longum subsp. infantis CBT BT1 had a good ability to utilize GOS. Strains CBT BT1 and CBT BF3 utilized 2'-fucosyllactose well and B. bifidum CBT BF3 had an ability to utilize 3'-sialyllactose as well as 2'-fucosyllactose. Vitamin B biosynthesis tests showed that the Bifidobacterium strains produced folate at very low concentrations even in the presence of 4-aminobenzoic acid as the precursor. However, all Bifidobacterium strains highly produced nicotinate in blood liver and SM7 media supplemented with nicotinamide as the precursor. B. bifidum CBT BF3 also highly produced riboflavin in media supplemented with guanine as the precursor. This study suggests that the Bifidobacterium strains may have potential values as probiotics having abilities to utilize indigestible oligosaccharides and HMO and to produce vitamin B.
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KEYWORD
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Bifidobacterium, indigestible oligosaccharide utilization, infant feces, probiotics, vitamin B biosynthesis
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